Beijing, March 24, 2018 – It is well understood that nucleotides, as monomers of DNA and RNA, play an important role in all life forms. Nucleotides also play a central role in metabolism at a cellular level as they carry chemical energy in the form of the nucleoside triphosphates (ATP, GTP, CTP, and UTP) through the cells to supply energy for important cellular functions. In addition, nucleotides participate in cell signaling (e.g., cGMP) and are incorporated into important cofactors of enzymatic reactions (such as in coenzyme A).

Nucleotides are comprised of a nitrogen-containing base, a five-carbon sugar, and at least one phosphate group. Nucleotides are derived from nucleosides which are lacking the phosphate groups. Nucleosides and their analogs are of great interest for medicinal chemists for example as antiviral agents or anticancer agents with Sofosbuvir and Gemcitabine as the well-known examples.

The scheme below shows Deoxyadenosine monophosphate (dAMP) as an example for the general structure of a nucleotide:

Deoxyadenosine monophosphate (dAMP)

We have worked on many nucleoside analog projects over the past 10+ years at BioDuro and have gained considerable experience in their – often lengthy and complex – synthesis. Recently, as part of our continuous efforts to expand our capabilities and service offerings, we have added more expertise especially in the area of nucleotide synthesis and purification.

To further enhance our capabilities in this field, we have allocated analytical and preparative HPLCs equipped with special columns (C18 column, Dionex BioLC DNAPac PA-100 column and GE Healthcare HiPrep DEAE column, etc.) for this purpose. With this expertise and equipment in place, BioDuro now can offer a wide range of nucleoside/nucleotide chemistry, such as nucleoside modification and phosphorylation (from mono-phosphate up to tetra-phosphates) to synthesize and purify nucleotides and short oligonucleotides. Some illustrative schemes are shown below:

Illustrative Protocol for Purifying Nucleotides and Short Oligonucleotides

For more information on nucleoside and nucleotide chemistry, please contact us. 

Register Now!
Webinar: New Modality - Peptides Innovations and a Comprehensive Synthetic Peptide Drug Discovery Platform
June 19, 2024 at 10am ET / 07am PT / 04pm CET
View more
We use necessary cookies to ensure our website functions properly and to keep it secure. These necessary cookies must be enabled. By clicking “Accept All Cookies”, you agree to the storing of cookies on your device to enhance site navigation, analyze site usage, and assist in our marketing efforts. You can personalize or withdraw your consent at any moment by clicking on the link "Cookies Settings". For more information on how we use cookies, including the total lifespan of cookies and the identity of third parties intervening on our website, please consult the Privacy Notice available in the site footer.
Accept all