Advanced immunization technologies such as immunizing animals with antigen-coding DNA, surface antigen-expressing cells, or virus-like particle bringing target surface antigens, which are preferred alternatives for targeting antigens that are difficult to express or purify. Usually, animal immunization can produce high affinity antibodies, but the fusion efficiency of conventional method is relatively low. At BioDuro-Sundia we use electroporation for DNA immunization and fusion process to achieve better efficiency

Novel plasmid with enhancing elements regulating expression and immunogenicity, which could keep constant/high-level expression and help to break the tolerance induced by homology

Variable strategies for antigen expression in native conformation, including DNA-based self-expressing, recombinant expression on cell surface/VLP, and antigen overexpressing tumor cell lines. Multiple adjuvants at different stages in the whole immunization process help to induce strong immune responses

Co-expression with co-receptors or chaperones helps to keep correct modification and conformation, especially for antigens in membrane complexes or with heavily glycosylation